Liquid chromatography-mass spectrometry (LC-MS) is the enabling technology for global-scale analysis of proteins (proteomics). Nanoflow LC-MS (flow rate < 1 µL/min) is routinely used to achieve high sensitivity for identifying and quantifying thousands of peptides and proteins in small volumes of biological samples, but lacks robustness and throughput (i.e., speed). By contrast, microflow LC-MS (flow rate 1-50 µL/min) typically achieves higher throughput and robustness with relative standard deviation (RSD) < 20%, but lacks the sensitivity of nanoflow LC-MS. The Newomics award-winning silicon multinozzle emitters (M3 emitters) enable optimization of sensitivity, throughput, and robustness by splitting the microflow eluent evenly into multiple nanoflows at the emitter, thereby dramatically enhancing the ionization efficiency.
Microflow LC-Nanospray MS for Bottom-up Proteomics
In this Application Note, we demonstrate the application of our microflow LC-nanospray ESI-MS platform (MnESI-LC-MS) for bottom-up proteomics studies of HeLa whole cell lysates and human plasma samples.
Microflow LC-Nanospray MS for Targeted Proteomics
In this Application Note, we demonstrate the application of our microflow LC-nanospray ESI-MS platform (MnESI-LC-MS) for targeted proteomics studies of peptide mixtures spiked in human plasma digest matrix.