APPLICATIONS

Liquid chromatography-mass spectrometry (LC-MS) is the enabling technology for global-scale analysis of proteins (proteomics). Nanoflow LC-MS (flow rate < 1 µL/min) is routinely used to achieve high sensitivity for identifying and quantifying thousands of peptides and proteins in small volumes of biological samples, but lacks robustness and throughput (i.e., speed). By contrast, microflow LC-MS (flow rate 1-50 µL/min) typically achieves higher throughput and robustness with relative standard deviation (RSD) < 20%, but lacks the sensitivity of nanoflow LC-MS. The Newomics award-winning silicon multinozzle emitters (M3 emitters) enable optimization of sensitivity, throughput, and robustness by splitting the microflow eluent evenly into multiple nanoflows at the emitter, thereby dramatically enhancing the ionization efficiency.

Microflow LC-Nanospray MS for Bottom-up Proteomics

In this Application Note, we demonstrate the application of our microflow LC-nanospray ESI-MS platform (MnESI-LC-MS) for bottom-up proteomics studies of HeLa whole cell lysates and human plasma samples.

Microflow LC-Nanospray MS for Targeted Proteomics

In this Application Note, we demonstrate the application of our microflow LC-nanospray ESI-MS platform (MnESI-LC-MS) for targeted proteomics studies of peptide mixtures spiked in human plasma digest matrix.

Newomics M3 Emitters for Shotgun Lipidomics

In this Application Note, we demonstrate the application of Newomics M3 emitters for shotgun lipidomics, showing identification and quantification of around 600 lipid species in 5 microliters of human plasma samples.

M3 Emitters for Microflow LC-MS Analysis of Native Antibodies

In this Application Note, we demonstrate the application of Newomics M3 emitters for microflow LC-MS analysis of native monoclonal antibodies, achieving high-throughput, high-sensitivity and robustness while preserving their native states.

MnESI-MS Platform for LC-MS Analysis of Native Protein Complexes

This application note establishes a MnESI-MS platform for LC-MS analysis of labile native protein complexes, that achieves high throughput, sensitivity, and robustness using the Newomics MnESI source and M3 multinozzle emitters interfaced with a Thermo QE UHMR mass spectrometer.

MnESI-MS Platform for Sensitive and Robust Analysis of Intact Antibodies

In this Application Note, we demonstrate the MnESI-MS platform for sensitive and robust LC-MS analysis of intact antibodies, achieving over 10-fold improvement in sensitivity over conventional methods, with a LOQ of 10 ng/mL with NIST mAb and a CV of less than 5%.

MnESI-MS Platform for LC-MS Analysis of Native Antibodies

In this application note, we report the integration of Newomics MnESI source and Bruker maXis II UHR-QTOF mass spectrometer for native size exclusion chromatography-mass spectrometry, for characterization of intact antibodies.

Enhanced Sensitivity and Robustness Using a Microflow LC and Nanospray MS Platform for Small Molecules Analysis

In this application note, we demonstrate significant sensitivity improvement and rugged performance for analyzing small molecules (with pesticides as an example in this note), using the Newomics microflow-nanospray ESI-MS (MnESI-MS) platform.

MnESI-MS Platform for Sensitive Analysis of Oligonucleotides

High-flow ion-pairing reversed-phase LC-MS has become the new method of choice for oligonucleotide analysis. However, the current LC-MS practice typically has a low sensitivity for negative ionization mode and high contamination to the MS. Now with the Newomics MnESI-MS platform, it’s possible to achieve more than 30-fold sensitivity gain and lower contamination due to less ion-pairing reagent entering the MS.