Newomics is on a mission to enable precision medicine with tools and applications. Our new DuoESI system is an integrated front-end solution for superior microflow and nanoflow LCMS, for proteomics, metabolomics, single-cell omics, and many other emerging mass spectrometry applications.
High Sensitivity
Robustness
Throughput
DuoESI
Source
MEA
Chip
M3
Emitter
ASMS 2023
June 4 - 8 | Houston
The DuoESI platform is the first fully integrated front-end solution for superior microflow and nanoflow LC-MS. A nanoflow module for the MEA chip and a microflow module for the M3 emitter are included with the platform.
The DuoESI source is compatible with all mass spectrometers from Agilent, Bruker, Thermo Fisher, and Shimadzu.
Our proprietary MEA chip achieves performance in small-volume bottom-up proteomics on par with the top commercial columns. All this while introducing novel functionalities that are not feasible with current nanoflow LC column technologies.
It expands the capabilities of LC-MS and opens the door to new and exciting advancements in proteomics.
Our award-winning multinozzle M3 Emitter splits incoming flow into multiple smaller streams, dramatically enhancing ionization for microflow LC-MS. Our MEA chip monolithically integrates the M3 Emitter with an on-chip LC column, reducing dead volume and simplifying the connection for nanoflow LC-MS.
It enables new mass spectrometry applications for clinical diagnostics, biomarker discovery, drug development, food and environmental monitoring, forensic analysis, and more.
5 - 50x
more sensitive compared to conventional methods.
CV ≤ 5%
from 300 plasma injections.
< 1 min.
per sample. More than 60 samples per hour.
Compared to the conventional single-nozzle emitters, the M3 emitter from Newomics provides 3-5 times higher peptide signals in ESI-MS and more robust and stable spray across the LC gradient. This allows me to use microliter flow rate LC-MS for a large set of biological samples for biomarker discovery, and ensures the reproducible results and similar sensitivity as nanoLC-MS has.
The 8-nozzle emitter showed a 5-10-fold increase in sensitivity for protein therapeutics in human serum compared to the standard LC-MS (HESI source) at the flow rate of 5 μL/min. Preliminary analysis of replicate injections from the same samples showed an excellent CV by the measurement of peak area ratios and no additional carryover.
3X improvement in peak intensities was observed for the 8-nozzle emitter in comparison with the ESI sprayer for non-denaturing LC-MS. Moreover, the generally sharper peaks and charge envelope shifted towards higher m/z suggest a more nanospray-like emission and possibly better preservation of the native conformation.
From my tests, the detection of a mix of 10 peptides spiked in plasma extracts, was 5-6X more sensitive (and up to > 10X) with Newomics multi-nozzle emitter than with classical ESI source with a single-nozzle.
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