M3 Emitters
The M3 emitter has multiple nozzles working together to split a single microflow stream evenly into multiple nanoflows, thereby dramatically enhancing the ionization efficiency to achieve unprecedented sensitivity, robustness, and throughput.
The M3 emitter has multiple nozzles working together to split a single microflow stream evenly into multiple nanoflows, thereby dramatically enhancing the ionization efficiency to achieve unprecedented sensitivity, robustness, and throughput.
Flexible Configurations
Find the Perfect Fit For Your Project
8-nozzle, 10 µm ID
1-10 µL/min
Catalog #: E8N10MU01
8-nozzle, 20 µm ID
10-40 µL/min
Catalog #: E8N20MU01
5-nozzle, 20 µm ID
5-20 µL/min
Catalog #: E5N20MU01
Product Details
Features
- Extremely sensitive, over 10 fold sensitivity gain
- Robust, clog-resistance
- Ease of use, plug-and-play operation
- Different emitter sizes available from 5 μm ID to 30 μm ID
- A wide range of flow rates from nano to microflows
- Interfaced to diverse mass spectrometers
- Reusable
Specifications
| Parameter | Specifications |
|---|---|
| Catalog # | E8N10MU01 |
| Nozzles | 8 |
| Inner Diameter (ID) of Nozzle | 10 µm |
| Outer Diameter (OD) of Nozzle | 20 µm |
| Internal Swept Volume | 10 nL |
| Flow Rate | 1 - 10 µL/min |
| Parameter | Specifications |
|---|---|
| Catalog # | E8N20MU01 |
| Nozzles | 8 |
| Inner Diameter (ID) of Nozzle | 20 µm |
| Outer Diameter (OD) of Nozzle | 30 µm |
| Internal Swept Volume | 14 nL |
| Flow Rate | 10 - 40 µL/min |
| Parameter | Specifications |
|---|---|
| Catalog # | E5N20MU01 |
| Nozzles | 5 |
| Inner Diameter (ID) of Nozzle | 20 µm |
| Outer Diameter (OD) of Nozzle | 30 µm |
| Internal Swept Volume | 12 nL |
| Flow Rate | 5 - 20 µL/min |
Performance Highlight
Newomics MnESI-MS platform streamlines analysis of native antibodies, antibody drug conjugates, and large protein complexes. It achieves static nanoflow sensitivity and excellent data quality, allows for automated processing and higher throughput via LC/MS, and provides better preservation of protein native state during the ESI process.
SEC-LC/MS analysis of GroEL tetradecaner protein complex using the MnESI-MS platform with M3 Emitter at the microflow of 5 µL/min.
Newomics MnESI-MS platform facilitates nucleic acid analysis in a negative mode. It delivers increased sensitivity and higher charge state which allows for detection of larger oligonucleotides with longer sequence, and better identification with more complete fragmentation (with a higher charge state).
Ion-Pair Reversed Phase (IP-RP) LC/MS Analysis of 75-mer tRNA using the MnESI-MS platform with M3 Emitter at microflow. MnESI platform attains improved sensitivity and higher charge state and comparable data quality vs. high-flow LC/MS method.
M3 EMITTER AT MICROFLOW DELIVERS NANOFLOW SENSITIVITY
Operating with 150 μm I.D. column at 1.5 μL/min, M3 emitter achieves ~3-5-fold sensitivity increase compared to a conventional single nozzle emitter (Easy-Spray) operating with the same column and flow rate. Moreover, M3 emitter at microflow rate demonstrates similar sensitivity to a conventional emitter operating with a nanoflow column (75 μm I.D.) at 0.3 μL/min.
M3 EMITTER WITH MNESI-LC-MS DELIVERS BETTER ROBUSTNESS AND REPRODUCIBILITY OVER NANOFLOW LC-MS
25 injections of 1 μg human plasma digests are used to assess the robustness. Compared to nanoflow LC-MS, the average RSDs achieved by MnESI-LC-MS are around 50% smaller. Especially for those peptides eluted at low organic mobile phase (retention time < 20 min), MnESI-LC-MS achieves much lower RSD values than nanoflow LC-MS.
M3 EMITTER ENABLES > 40X SENSITIVITY GAIN OVER HIGH-FLOW LC-MS
Microflow LC-Nanospray MS with M3 emitter delivers more than a 40-fold sensitivity increase when compared to conventional highflow LC-MS.
MNESI-LC-MS WITH M3 EMITTER ENABLES HIGH-FLOW ROBUSTNESS
300 injections of human plasma digests are performed to test robustness. MnESI-LC-MS with M3 emitter achieves the average peak area intensity RSD of 5%, without using an internal standard to correct for MS signal variability. In contrast, the peak area intensity RSD for the high-flow LC-MS is about 6% on average.
Newomics MnESI-MS platform allows for sensitive identification and quantitation of lipids in human plasma samples. It achieves increased sensitivity and assay reproducibility and allows hundreds of lipid species to be measured from a drop of blood.
MnESI-MS platform with M3 emitter achieves enhanced sensitivity (2.5-fold) vs. conventional nanoflow method using single nozzle emitters in shotgun lipidomics of human plasma samples.
