Application Proteomics
Product Newomics MnESI Source for Thermo Scientific Legacy Mass Spectrometers; M3 Emitter
Instrumentation Thermo Scientific PepMap C18 columns on a Thermo Scientific Exactive Plus Mass Spectrometer
Samples Thermo Scientific Pierce HeLa Protein Digest Standard; human plasma digest prepared by Thermo Scientific EasyPep Mini MS Sample Prep Kit
Performance 3.8 sensitivity gain
Introduction
Liquid chromatography-mass spectrometry (LC-MS) is the enabling technology for global-scale analysis of proteins (proteomics). Nanoflow LC-MS (flow rate < 1 µL/min) is routinely used to achieve high sensitivity for identifying and quantifying thousands of peptides and proteins in small volumes of biological samples, but lacks robustness and throughput (i.e., speed). By contrast, microflow LC-MS ( ow rate 1-50 µL/min) typically achieves higher … (Read More)
Application Proteomics
Product MnESI Source for Thermo Scientific Legacy Mass Spectrometers; Flow Splitting Kit for Microflow LC-MS; M3 Emitter
Instrumentation Thermo Fisher PepMap™ C18 columns on a Quantiva™ mass spectrometer for SRM analysis and a Q Exactive™ Plus mass spectrometer for PRM analysis
Samples Human plasma
Performance 50-fold higher than high-flow and 2.3-fold higher than conventional microflow LC-MS for proteomics.
Introduction
Liquid chromatography-mass spectrometry (LC-MS) is the enabling technology for the global-scale analysis of proteins (proteomics). In order to identify and quantify peptides and proteins in small volumes of biological samples, nanoflow LC-MS (flow rate < 1 mL/min) is routinely used in order to achieve high sensitivity. However, nanoflow LC-MS lacks robustness and throughput (i.e., speed). On the other hand, high-flow LC-MS … (Read More)
Application Lipidomics
Product Newomics M3 multinozzle Emitter
Instrumentation Thermo Fisher TSQ Quantiva™ mass spectrometer
Samples Pooled human plasma samples (NIST SRM1950)
Performance ~7.5% CV; LOD (S/N=3)=0.17 pmole/uL; 2-fold sensitivity increase
Introduction
Shotgun lipidomics by ESI-MS is widely used for the global analysis of lipids in biological samples such as human plasma. Direct-infusion ESI-MS by nanoflow (flow rate < 1 µL/min) is routinely performed to achieve high sensitivity for identifying and quantifying hundreds of lipid species in small volumes of samples. Current single-nozzle emitter technologies including PicoTip1•2•3•A and NanoTip5 have been utilized for … (Read More)
