Chromatography is often the slowest step between your sample and your answer. Separation, equilibration, and re-equilibration can add significant time to an LC-MS analysis, even when chromatographic separation is unnecessary. Switching between analytical workflows can also require different LC columns and methods.
Our new application note demonstrates how UniDirect™ combines automated direct infusion with microflow multispray ionization for rapid, reproducible, chromatography-free biopharmaceutical analysis. A single platform supports peptide mapping, denatured intact MS, and native MS for monoclonal antibody (mAb) analysis.
Key Performance Highlights:
- High throughput: 96 wells analyzed in under 224 minutes, corresponding to a projected throughput of more than 576 samples/day.
- High reproducibility: Peptide-ion CVs of 6.8% and 7.5% across 88 sample wells.
- Low carryover: Approximately 1% sample-to-blank carryover with automated washing.
- High sensitivity: BSA digest peptides detected at 0.1 ng/µL, the lowest concentration tested.
The full application note includes the automated workflow, experimental methods, concentration-response curves, 96-well reproducibility data, and intact MS, native MS, and peptide-mapping results for NISTmAb.
Read Application Note