UniESI Source

Using a single ion source interfaced with mass spectrometers, you can achieve ultrasensitive single-cell proteomics, and reproducible high-throughput quantitation of proteins or small molecules. The UniESI Source is a game-changing product. A truly open-source end-to-end solution that puts an end to juggling 3-4 ion sources with different tubing connections on the same mass spectrometer.

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Using a single ion source interfaced with mass spectrometers, you can achieve ultrasensitive single-cell proteomics, and reproducible high-throughput quantitation of proteins or small molecules. The UniESI Source is a game-changing product. A truly open-source end-to-end solution that puts an end to juggling 3-4 ion sources with different tubing connections on the same mass spectrometer.

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Enhanced Workflow

Built for Superior Microflow and Nanoflow LC-MS

Sensitivity

5 - 50x

more sensitive compared to conventional methods.

 

Robustness

CV ≤ 5%

from 300 plasma injections.

 

Throughput

< 1 min.

per sample for direct infusion ESI. More than 60 samples per hour.

 

Product Configurations

Compatible with Agilent and Thermo Fisher Mass Spectrometers

IS-A01U

for Agilent MS

Compatible with: 6500 Series Q-TOF, 6400 Series QQQ, 6200 Series TOF, and all other Agilent MS

IS-T01U

for Thermo Fisher MS (New Generation)

Compatible with: Orbitrap Exploris (120, 240, 480), Orbitrap Astral, Orbitrap Tribrid Series (Fusion, Lumos, ID-X, Eclipse, Ascend), TSQ Series II (Endura, Quantiva, Altis, Quantis, Fortis)

Product Details

  • Features
  • Specifications
  • Literature

    • Features

    • The first ion source with multiple swappable microflow and nanoflow modules
    • Compatible with all mass spectrometers from Thermo, Bruker, Agilent, Shimadzu, and Waters
    • Plug-and-play platform for Newomics M3 emitters and MEA chips
    • Plug-and-play platform for third-party columns and other LCMS consumables
    • A universal, unified ion source for low-flow electrospray ionization mass spectrometry

    Specifications

    ParameterSpecifications
    Ionization Mode

    Atmospheric pressure electrospray ionization (ESI), both positive and negative mode.

    ESI Probe

    Newomics M3 Emitter and MEA Chip (refer to M3 Emitter and MEA Chip specification)

    Open or Closed System

    Semi-closed source

    Flow Rate

    Nano (20 – 1000 nL/min), for nanoMEA chips
    Micro (1 – 50 µL/min), for M3 emitters and microMEA chips

    Spray Angle

    Fixed angle of 25° for M3 emitters
    0° angle for nanoMEA chips

    Capillary Voltage

    0 – 6 kV

    Spray Current

    0 – 10 µA, max. 50µA

    Sheath Gas Flow Setting

    0 – 30 (arbitrary units)

    Auxiliary Gas Flow Setting

    0 – 20 (arbitrary units, optional)

    Translational Stage

    Linear XY-axis manual stage, ±5 mm travel distance, 30 µm travel accuracy

    Camera

    1.3M pixels, 20x - 90x magnification rate, color CMOS sensor, up to 30 fps frame rate

    Light Source

    Cool white (9000K) 5 mm round LED light, luminous intensity 46400 mcd

    Connection

    Face seal, finger-tight 10-32 threaded fittings including Thermo nanoViper, IDEX MarvelX and MarvelXACT, Waters ZenFit, Phenomenex SecurityLink, VICI Cheminert C360IZR1, and nanoConnect

    Unit Dimension

    Width: 25 cm (9.8 in.)
    Height: 17.5 cm (6.9 in.)
    Depth: 15 cm (5.9 in.)

    Unit Weight

    3 kg (6.6 lbs)

    ModelSpecifications
    Catalog # IS-A01U

    UniESI Source for Agilent Mass Spectrometers:
    6500 Series Q-TOF, 6400 Series QQQ, 6200 Series TOF, and all other Agilent MS

    Catalog # IS-T01U

    UniESI Source for Thermo Fisher New Generation Mass Spectrometers:
    Orbitrap Exploris (120, 240, 480)
    Orbitrap Tribrid Series (Fusion, Lumos, ID-X, Eclipse, Ascend)
    TSQ Series II (Endura, Quantiva, Altis, Quantis, Fortis)

    Catalog # IS-T02U

    UniESI Source for Thermo Fisher Legacy Mass Spectrometers:
    Q Exactive Series (QE Focus, QE, QE Plus, QE HF, QE HFX, QE UHMR)
    Exactive Series (Exactive Plus, Exactive Plus EMR)
    Orbitrap Elite LTQ Series (LTQ XL, LTQ Orbitrap XL, Velos Pro)
    LCQ Fleet TSQ Series (Quantum, Vantage)

    Literature