A Versatile, Sensitive, and Robust Native LC-MS Platform for Intact Mass Analysis of Protein Drugs
Yan et. al., Regeneron, J. Am. Soc. Mass. Spectrum. 2020, 31, 10, 2171-2179
Very impressive work published recently by Regeneron. They describe the development of an integrated LC-MS platform for native protein drug characterizations, enabled by Newomics multinozzle emitters. In particular, the developed platform can tolerate a wide range of LC flow rates and high salt concentrations, which are critical for accommodating different nLC methods. The platform also achieves online charge-reduction native MS, which improves the characterization of both heterogeneous and labile biomolecules.