Improving lipidomics profiling of 3D renal carcinoma by microflow chromatography coupled to multinozzle electrospray ionization

Abstract

Most bioanalytical laboratories rely on LC-MS-based workflows operating at analytical flow rates of 0.2-1mL/min with the use of electrospray to produce charged ions for MS detection. Profiling approaches in lipidomics require a wide coverage while keeping moderate run times (< 30min) to enable the measurement of sufficient replicates and conditions to deconvolute biological effects. Nanoflow techniques are well recognized as offering major improvements in coverage but require long run times and are prone to clogging and damage. Operating at microflow regimen (1-100 µL/min) enables an easier operation but the gains in sensitivity do not justify the transition from conventional analytical flow methods. In this webinar, we will present how we combined microflow with multinozzle electrospray ionization to obtain nano-ionization sensitivity while achieving comparable run times as analytical flow methods. We will explain some practical aspects and illustrate the potential of this setup in the profiling of renal cancer cells subjected to combination chemotherapy.