Please fill out this form to access this resource
Abstract
Native ion mobility–mass spectrometry (nIM-MS) and collision-induced unfolding (CIU) enable detailed analysis of protein structure and stability. Static nano-electrospray ionization (nESI) emitters are the standard approach for nIM-MS experiments but require extensive manual handling and operate at flowrates too low to accommodate many biopharmaceutical applications. Here, we combine multi-nozzle nESI (mnESI) emitters with both commercial liquid chromatography hardware and high-speed robotic sampling methods to automate sample introduction and deliver nESI-like spray at analytical flow rates, enabling fully automated nMS and CIU workflows. The automated platform is then used to classify hinge-engineered antibodies as agonistic or non-agonistic, with classification accuracy comparable to that of low-throughput static nESI.
