Affinity-based chromatography and native mass spectrometry strategies for studying attribute-and-function relationships in monoclonal antibodies

Wednesday, August 6th, 2025 at 10 AM PT

Abstract

Evaluating critical quality attributes (CQAs) is essential in developing therapeutic monoclonal antibodies (mAbs). CQAs, such as attributes affecting target binding or Fc receptor engagement, directly influence drug safety and efficacy. In this webinar, we introduce two innovative techniques for assessing key modifications in mAb drug candidates: (1) enrichment based on target-affinity and (2) affinity-resolved size exclusion chromatography coupled with native mass spectrometry (AR-SEC-MS). We will discuss the development of these methods and their applications in studying the relationship between mAb attributes and functions.

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Speaker

Yuetian Yan, PhD

Associate Director, Analytical Chemistry
Regeneron Pharmaceuticals, Inc.

Yuetian Yan is an Associate Director in the Analytical Chemistry department at Regeneron Pharmaceuticals, Inc. Her work focuses on developing LC/MS methods to study therapeutic proteins, supporting drug development and regulatory processes.

Speaker

Jennifer Nguyen, PhD

Sr. Principal Scientist
Regeneron Pharmaceuticals, Inc.

Jennifer Nguyen is a Sr. Principal Scientist in the Protein Biochemistry department at Regeneron Pharmaceuticals, Inc. She leads a team which provides late-stage biophysical and biochemical characterization of biologics, including therapeutic antibodies and AAVs.